Hi friends, today we are going to discuss you the latest Syllabus of Class XII BioTechnology Syllabus. As you know very well that Central Board of Secondary Education (CBSE) has its own curriculum and they hold the supreme authority to change it further for the benefit of students.
So it’s always a wise decision to keep an eye on what happening around with the subject you are going to learn. This year CBSE made little bit changes in class XII BioTechnology Syllabus. Let us have a look at the updated CBSE class XII BioTechnology Syllabus for the academic year 2017-18
CBSE class XII Chemistry Syllabus (2018-19 Session)
CLASS XII (2017-18)
One Paper Max. Marks 70+30
Time: 3 hrs.
|Units||No. of Periods||Marks|
|Unit-V||Protein and Gene Manipulation||100||40|
|Unit-VI||Cell Culture and Genetic Manipulation||80||30|
Time: 3 hrs. Total Marks: 70
Unit-V Protein and Gene Manipulation (40 Marks, 100 Periods)
Chapter-1: Recombinant DNA Technology
Introduction, Tools of Recombinant DNA technology, Making Recombinant DNA, Introduction of Recombinant DNA into Host Cells, Identification of recombinants, Polymerase chain reaction (PCR), Hybridization techniques, DNA library, DNA sequencing, Site-directed mutagenesis
Chapter-2: Protein Structure and Engineering
Introduction to the World of Proteins, 3-D Shape of Proteins, Structure-function relationship in Proteins, Purification of proteins, Characterization of proteins, Protein-Based products, Designing proteins (protein engineering)
Chapter-3: Genomics, Proteomics, and Bioinformatics
Introduction, Genome sequencing projects, Gene prediction and Counting, Genome similarity, SNPs and Comparative Genomics, Functional genomics, Proteomics, History of bioinformatics, Sequences, and Nomenclature, Information sources, Analysis using bioinformatics Tools
Unit-VI Cell Culture and Genetic Manipulation (30 Marks, 80 Periods)
Chapter-1: Microbial Cell Culture and Its Applications
Introduction, Microbial culture techniques, Measurement and kinetics of microbial growth, Scale-up of a microbial process, Isolation of microbial products, Strain isolation, improvement and Preservation, Applications of microbial culture technology, Biosafety Issues in microbial technology
Chapter-2: Plant Cell Culture and Applications
Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Gene transfer methods in plants, Transgenic plants with beneficial traits, Biosafety of Transgenic Plants.
Chapter-3: Animal Cell Culture and Applications
Introduction, Animal cell culture techniques, Characterisation of cell lines, Methods of gene delivery into cells, Scale-up of animal culture process, Applications of animal cell culture, Stem cell technology, Tissue engineering
PRACTICALS (30 Marks, 60 Periods)
Note: Every student will be required to do the following experiments during the academic session.
List of Experiments
1. Use of special equipment in biotechnology experiments.
2. Isolation of bacterial plasmid DNA
3. Detection of DNA by gel electrophoreses
4. Isolation of Genomic DNA (CTAB method)
5. Estimation of DNA
6. Bacterial transformation using any plasmid
7. Restriction digestion of plasmid DNA & its analysis by gel electrophoreses
8. Isolation of bacterial grom curd & staining of bacteria
9. Cell viability assay
10. Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from the internet, analyze it and comment on it
11. Reading of a DNA sequencing gel to arrive at the sequence
12. Project work
Scheme of Evaluation:
Time: 3 Hours (Max. Marks 30)
The scheme of evaluation at the end of the session will be as under:
|A||Two experiments||6+6 (only one computer-based practical)|
|Viva on Practicals||04|
|Viva on project||05|
1. A Text Book of Biotechnology – Class XI: Published by CBSE, New Delhi
2. A Laboratory Manual of Biotechnology – Class XI: Published by CBSE, New Delhi
3. A Text Book of Biotechnology – Class XII: Published by CBSE, New Delhi
4. A Laboratory Manual of Biotechnology – Class XII: Published by CBSE, New Delhi
|QUESTION PAPER DESIGN|
|S. No.||Typology of Questions||Very Short Answer (VSA) (1 mark)||Short Answer-I (SA-I) (2 marks)||Short Answer-II (SA-II) (3 marks)||Long Answer (L.A.) (5 marks)||Total Marks||% Weightage|
|02||Conceptual Understanding (Application and Reasoning based)||3||4||8||1||40||57%|
|03||Higher Order Thinking Skills (HOTS)||1||2||–||–||05||07%|
Total No. of Questions = 28
1. No chapter wise weightage. Care to be taken to cover all the chapters.
2. The above template is only a sample. Suitable internal variations may be made for generating similar templates
keeping the overall weightage to the different form of questions and typology of questions same.